Appendix Worksheets: Multi-Omics Analysis of Tissue Taphonomy for Forensics and Bioarchaeological Applications

Excel Workbook Containing Appendix Worksheets for PhD Thesis:

Appendix Sheets 3.1: Recovery of Decomposed Pig Leg Metabolite Peak IDs using MultiQuant and MS-DIAL software

Metabolites were extracted from both skin and muscle layers of a pig leg over seven timepoints, from initial zero hour (0HR) to of a 42 days (42D), and ran on a SCIEX 6600 TripleTOF Q-TOF mass spectrometer. Data acquired using SWATH-DIA were processed using both commercial (SCIEX’s MulitQuant 3.0.2, ins. ref ) and open-source (MS-DIAL 4.9) software. Peaks from MS1 and MS2 were matched against a metabolite library of 235 standards in MultiQuant and scored using a custom VBA macro (a). MS2 (b) and MS1 (c) peaks were processed independently after MS-DIAL, with sorting and filtering based on identification scores. In all cases, green results show a high confidence of identification, yellow a lower confidence and red indicates missing IDs.

Appendix Sheets 3.2: Unique Compounds Recovered from Decomposed Pig Leg Lipids identified through Progenesis software from Mass Spectrometry ran in Positive (a) and Negative Mode (b)

Appendix Sheet 4.1 Observations during Modelling and Extraction of Decomposing Porcine Leg Skeletal Muscle (MU) and Skin (SK)

Appendix Sheet 4.2 Protein Mass Spectrometry Sample Digestion and Manifest

Appendix Sheet 4.3 DNA Purification