Extended data - Increased in vivo transduction of AAV-9 cargo in Alport podocytes
Extended data for manuscript - Increased in vivo transduction of AAV-9 cargo in Alport podocytes
This project contains the following extended data:
Extended data S. Method - Picrosirius staining and quantification pipeline
Extended data S. Figure 1- AAV9-GFP-WPRE reporter plasmid. Circular map of the AAV9-GFP reporter construct with the regulatory elements. (Aldevron plasmid # 5069-10; https://www.aldevron.com/catalog-products/viral-vector-plasmids/pald-aav)
Extended data S. Figure 2 - Image panel of isolated mouse glomeruli transduced with AAV9-GFP. Images of 10 wild type glomeruli isolated from 8-week-old mice (control, no AAV9-GFP); 12 wild type and Col4a5 KO glomeruli transduced with AAV9-GFP. Scale bar= 25 µm.
Extended data S. Figure 3 - Representative images of liver cross-sections from 12-week-old mice injected with AAV9-GFP. A) Representative images of liver cross-sections from 12-week-old mice injected with 1.5 x 1014 vg/kg (high dose) of AAV9-GFP. Quantification of GFP intensity in the liver. Scale bar= 1000 µm. Zoomed in images, scale bar= 100 µm. B) Schematic representation and quantification of liver stained with picrosirius red.
