Imaging Mass Cytometry Data from Static and Growing Vestibular Schwannoma Brain Tumours

Four static (<20% volume change per year) and five growing (≥20% volume change per year) vestibular schwannoma (VS) brain tumours were run on the Hyperion Imaging Mass Cytometry System. Individual denoised image files for each metal-conjugated antibody channel are available as 32-bit TIFF files per region of interest (ROI) in the 'images.zip' folder along with the cell 'masks.zip' generated from single cell segmentation. Each pixel is 1 um^2, and pixel values correspond to the total counts for that metal in that pixel. The Bodenmiller pipeline was used to generate the cell table (cell_table.csv), in which each cell is one row. The columns represent the mean marker expression for that channel, the cells' location (Location_Center_X, Location_Center_Y), the region name (ROI), or cell ID within that ROI (ObjectNumber). The details for the different samples can be found in the ROI tab of the Cases.xls file. Details of whether the regions of interest (ROI) came from either static or growing VS are detailed in static_growing.csv.

cases.xls - Clinical information about the VS cases

cell_table.csv - Single-cell data, with each row for one cell

images.zip - Zipped and denoised IMC images, with each channel being an individual .tiff file, with each region of interest (ROI) having its own folder.

masks.zip - Cell masks used to define single cells as regions for single cell expression.

static_growing.csv - Details of whether the regions of interest (ROI) came from either static or growing VS as defined by a volumetric change per year of <20% or ≥20%, respectively.

Sharing/Access: This data was generated by Gregory, Haley, Jones et al. at the University of Manchester, please cite our publication if you use these data.