Figure 1a The natural reaction of QueF in vivo from preQ0 to preQ1 drawn with ChemDraw 21.
Figure 1b Schematic representation of an antiparallel β-sheet formation of the Y5Y fusion protein drawn with ChemDraw 21.
Figure 1c Homology model of preQ0 (green sticks) docked in QueF (black ribbon) with a short serine linker (pink) added followed by the Y5Y chain (red). Model created using PDB 4GHM and I-TASSER in PyMOL 2.0.
Funding
Manchester-Bangor DTP2
Biotechnology and Biological Sciences Research Council