<p>Figure 4. Energy profiles calculated by DFT for the four stages of QueF reduction of its natural substrate preQ<sub>0</sub> compared to the target nitrile BnCN. The rate-limiting step is the final hydride transfer. Data for preQ<sub>0</sub> from Hirao et al.</p>
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<p>Chemical drawings done in ChemDraw 21.0.</p>
Funding
Manchester-Bangor DTP2
Biotechnology and Biological Sciences Research Council