Detailed protocols supporting DExCon study

Gemperle, Jakub

doi:10.48420/16878859.v1

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Detailed protocols supporting DExCon study

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posted on 2021-12-06, 09:44 authored by Jakub GemperleJakub Gemperle

Detailed protocols supporting DExCon study. From preparation of ssDNA for CRISPR mediated knock in; delivery of CRISPR/Cas9 as RNP via magnetofection and screening.

Protocol for Spheroid invasion assay “on a chip" is included as well.

Funding

Endosome positioning in tumour-stroma interactions

European Commission

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DExCon, DExogron, LUXon: on-demand expression control of endogenous genes reveals dynamics of Rab11 family members

School of Biological Sciences

Keywords

protocols CRISPR gene editing technology spheroids invasion analysis Single strand DNA Biological Techniques

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CC BY 4.0

Detailed protocols supporting DExCon study

Funding

Endosome positioning in tumour-stroma interactions

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Read the peer-reviewed publication

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