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Detailed protocols supporting DExCon study

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posted on 2021-12-06, 09:44 authored by Jakub GemperleJakub Gemperle
Detailed protocols supporting DExCon study. From preparation of ssDNA for CRISPR mediated knock in; delivery of CRISPR/Cas9 as RNP via magnetofection and screening.
Protocol for Spheroid invasion assay “on a chip" is included as well.

Funding

Endosome positioning in tumour-stroma interactions

European Commission

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    School of Biological Sciences

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    Keywords

    protocolsCRISPR gene editing technologyspheroidsinvasion analysisSingle strand DNABiological Techniques

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