Supplementary Video 3 - L. monocytogenes response to spent media
<p> <strong>Supplementary Video 3. </strong><em><strong>L. monocytogenes</strong></em><strong> exposed to spent host cell media.</strong> 1.0 x 107 Lm-dsRed-<em>PactA</em>-GFP was incubated for 2 hours at 37°C in spent media (bottom) (retrieved as described in Section 2.6.1). Representative video of 3 biological replicate experiment show upregulation of PrfA-regulated virulence genes via <em>PactA</em>-GFP (green) expression and the formation of aggregates occurs when exposed to spent media, but not fresh media. Scale bar 10 μm.</p>
