Supplementary Video 3 - Spent Media Assay.mov (43.06 MB)
Supplementary Video 3 - L. monocytogenes response to spent media
Supplementary Video 3. L. monocytogenes exposed to spent host cell media. 1.0 x 107 Lm-dsRed-PactA-GFP was incubated for 2 hours at 37°C in spent media (bottom) (retrieved as described in Section 2.6.1). Representative video of 3 biological replicate experiment show upregulation of PrfA-regulated virulence genes via PactA-GFP (green) expression and the formation of aggregates occurs when exposed to spent media, but not fresh media. Scale bar 10 μm.
