<p> </p>
<p>Supplementary Video 4. <em>L. monocytogenes</em> exposed to fresh media.</p>
<p>1.0 x 107 Lm<em>-</em>dsRed<em>-</em>P<em>actA-</em>GFP was incubated for 2 hours at 37°C in fresh media (bottom). Representative video of 3 biological replicate experiment show upregulation of PrfA-regulated virulence genes via <em>PactA-</em>GFP (green) expression but no aggregation occurs when exposed to fresh media. Scale bar 20 μm.</p>