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<p><strong>Movie 1:</strong> Maximum intensity projection of a representative embryo showing <em>hb</em> translation sites (grey) and Histone-RFP (magenta) through nc11-14. Embryos are from females with single copies of <em>scFv-mNG-NLS</em> and <em>His-RFP</em> crossed to males with the <em>hbP2>SunTag-hb </em>transgene, and are imaged with a 40x objective, 1.1x optical zoom and 46 sec time resolution between frames. Time stamp is in min:sec. </p>
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<p><strong>Movie 2: </strong>Single plane time-lapse experiment visualising plasma membrane tethered MCP-mRuby3-CAAX and bound<em> hbP2>SunTag-hb</em> mRNAs (magenta) and translation sites or mature proteins bound by scFv-mNG-NLS (green). Embryos are from females carrying single copies of the <em>scFv-NG</em> and <em>tdMCP-Ruby3-CAAX</em> transgenes crossed to <em>SunTag-hb-MS2</em> transgenic males. Imaged with a 63x objective, 8x optical zoom, and a time resolution of ~2 sec per frame. Time stamp is in min:sec. </p>
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<p><strong>Movie 3: </strong>Single plane time-lapse experiment showing a single protein being released from a translation site (green, scFv-mNG-NLS) and diffusing away from the mRNA (magenta, tdMCP-mRuby3-CAAX) and site of translation. The embryo genotype and imaging settings are as described for movie 3, except the time resolution is ~1.7 sec per frame. </p>
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<p><strong>Movie 4: </strong>Single plane time-lapse experiment visualising translation termination. An active translation site site (green, scFv-NG) terminates translation and a single protein diffuses away from the mRNA molecule (magenta, MCP-mRuby3-CAAX). The embryo genotype and imaging settings are as for Movie 2.</p>
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<p><strong>Movie 5: </strong>Enlarged view of Movie 2, highlighting independent translation sites (green) fusing and separating over time. </p>
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<p><strong>Movie 6:</strong> Maximum intensity projection of another representative embryo showing <em>hb</em> translation sites (grey) and Histone-RFP (magenta) through nc11-14. Embryos are from females with single copies of <em>scFv-mNG-NLS</em> and <em>His-RFP</em> crossed to males with the<em> hbP2>SunTag-hb </em>transgene, and are imaged with a 40x objective, 1.1x optical zoom and 46 sec time resolution between frames. Time stamp is in min:sec. </p>
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<p><strong>Movie 7:</strong> Maximum intensity projection of a representative embryo showing <em>hb</em> translation sites (green) and Histone-RFP (magenta) through nc11-14. Embryos are from females with single copies of <em>scFv-mNG-NLS</em> and <em>His-RFP</em> crossed to males with the<em> hbP2>SunTag-hb-SV40 </em>transgene, and are imaged with a 40x objective, 1.1x optical zoom and 46 sec time resolution between frames. Time stamp is in min:sec. </p>
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