Macro for spheroid invasion assay including protocol

Spheroid invasion assay “on a chip”. 3D micropatterning is made using 3D Petri dishes (microtissues; #12-81) which generates 81 spheroids of uniform sizes and also served as starting point for our spheroid invasion assay “on a chip”. Macro is able to analyse area of invading cells directly from brightfield image (see the protocol, analysis part)