LOCUS pJET1.2_22_TRE3GS-NG2-R 4742 bp ds-DNA circular 23-APR-2021 DEFINITION . KEYWORDS "Name:pJET1.2/blunt 07_NG2-Rab11A" "Plasmid:pJET1.2/blunt 07_NG2-Rab11A" "Strain/Frozen stock" "Resistance (bacteria):Amp" "Gene or insert" "Owner" "Location:blue box" "Sub-location" "Location details" "Backbone" "Technical details" "Created by" "3' Cloning site - Enzyme Used" "3' primer" "5' Cloning site - Enzyme Used" "5' primer" FEATURES Location/Qualifiers primer 33..52 /label="F_UNIVERSAL" /note="sequence: AGCTGATAATACGACTCACTATAGGG" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 100..125 /label="T7 promoter preceded by 6 arbitrary nucleotides for optimal in vitro transcription" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 100..125 /label="F_UNIVERSAL" /note="sequence: AGCTGATAATACGACTCACTATAGGG" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 100..431 /label="ORDER_5HDR" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 125..302 /label="intron" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer complement(409..427) /label="58_R_Rab11A-EcoRI" /note="sequence: CGtaGAATTCGCGGCCGAGGAGCGAAAGG" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer complement(409..437) /label="60" /note="sequence: tcaggtccacggtctcagagaagccCATTGCGCGGCCGAGGAGCGAAAGG" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 431..802 /label="TRE3GS" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer 800..822 /label="57_F_Rab11A-EcoRI" /note="sequence: GatcGAATTCGCAATGGGATCGGGTGGGACTAG" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 CDS 803..1603 /label="Translation 803-1603" misc_feature 806..826 /label="LinkerS1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(806..826) /label="R_uniGSlink_SpeI" /note="sequence: GCCACTAGTCCCACCCGATCC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 806..830 /label="59" /note="sequence: gatgacccagcagaagacaagctccGGATCGGGTGGGACTAGTGGCGTGT" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 806..1552 /label="ORDER_specific (Invitrogen optimalizovat!)" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 827..1531 /label="NeonGren_optimalized" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer complement(1509..1531) /label="53_R_SacI_NG2" /note="sequence: GTAGAGCTCCTTGTACAGCTCGTCCATGCCCA" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 1532..1552 /label="linkerS2" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 1532..1867 /label="ORDER_3HDR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 1550..1555 /label="mut PAM" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1550..1586 /label="Rab11exon1" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e ORIGIN 1 CACTTGTGCC TGAACACCAT ATCCATCCGG CGTAATACGA CTCACTATAG GGAGAGCGGC 61 CGCCAGATCT TCCGGATGGC TCGAGTTTTT CAGCAAGATA GCTGATAATA CGACTCACTA 121 TAGGGACTAT GGTAGCTAGG AGTTCCAGGA CTCAGTTTCC CCTTTGAGCC TCCTTTAGCG 181 ACTAAAGCTT GAAGCCCCAC GCATCTCGAC TCTCGCGCAC ACCGCCCTTG TTGGGCTCAG 241 GGGCGGGGCG CCGCCCCCGG AAGTACTTCC CCTTAAAGGC TGGGGCCTGC CGGAAATGGC 301 GCAGCGGCAG GGAGGGGCTC TTCACCCAGT CCGGCAGTTG AAGCTCGGCG CTCGGGTTAC 361 CCCTGCAGCG ACGCCCCCTG GTCCCACAGA TACCACTGCT GCTCCCGCCC TTTCGCTCCT 421 CGGCCGCGCA GAATTCTTTA CTCCCTATCA GTGATAGAGA ACGTATGAAG AGTTTACTCC 481 CTATCAGTGA TAGAGAACGT ATGCAGACTT TACTCCCTAT CAGTGATAGA GAACGTATAA 541 GGAGTTTACT CCCTATCAGT GATAGAGAAC GTATGACCAG TTTACTCCCT ATCAGTGATA 601 GAGAACGTAT CTACAGTTTA CTCCCTATCA GTGATAGAGA ACGTATATCC AGTTTACTCC 661 CTATCAGTGA TAGAGAACGT ATAAGCTTTG CTTATGTAAA CCAGGGCGCC TATAAAAGAG 721 TGCTGATTTT TTGAGTAAAC TTCAATTCCA CAACACTTTT GTCTTATACC AACTTTCCGT 781 ACCACTTCCT ACCCTCGTAA AGATGGGATC GGGTGGGACT AGTGGCGTGT CTAAGGGCGA 841 AGAGGACAAT ATGGCCAGCC TGCCTGCCAC ACACGAGCTG CACATCTTCG GCAGCATCAA 901 CGGCGTGGAC TTCGACATGG TCGGACAAGG CACCGGCAAT CCCAACGACG GCTACGAGGA 961 ACTGAACCTG AAGTCCACCA AGGGCGACCT GCAGTTCAGC CCCTGGATTC TGGTGCCTCA 1021 CATCGGCTAC GGCTTCCACC AGTACCTGCC TTATCCTGAC GGCATGAGCC CTTTCCAGGC 1081 CGCCATGGTT GATGGCTCTG GCTACCAGGT GCACCGGACC ATGCAGTTTG AAGATGGCGC 1141 CAGCCTGACC GTGAACTACC GGTACACATA CGAGGGCAGC CACATCAAAG GCGAGGCCCA 1201 AGTGAAAGGC ACAGGCTTCC CTGCTGATGG CCCCGTGATG ACCAATTCTC TGACAGCCGC 1261 CGACTGGTGC AGAAGCAAGA AAACATACCC CAACGACAAG ACCATCATCA GCACCTTCAA 1321 GTGGTCCTAC ACCACCGGCA ACGGCAAGCG GTACAGAAGC ACCGCCAGAA CCACCTACAC 1381 CTTCGCCAAA CCTATGGCCG CCAACTACCT GAAGAACCAG CCTATGTACG TGTTCCGCAA 1441 GACCGAGCTG AAGCACTCCA AGACAGAACT GAACTTCAAA GAGTGGCAGA AAGCCTTCAC 1501 CGACGTGATG GGCATGGACG AGCTGTACAA GGGAACCGGT GCTGGAAGTG GTACACGCGA 1561 CGACGAGTAC GACTACCTCT TTAAAGGTGA GGCCATGGGC TCTCGCACTC TACACAGTCC 1621 TCGTTCGGGG ACCCGGGCCA CTCCCGGTGG ACCCTCGTGC CGGCCACCCC TGCACTGATA 1681 TAGGCCTCCC TCAGCCCTTC CTTTTTGTGC GGTTCCGTCT CCTACCCAGC TCAGCCTCTT 1741 CTCCCCCGCT CAGACAGGGG TCCCCATCAC ATGCCGCTCT CTGAGCGACC TCTCCATAGG 1801 CCTTCGCTGG CCTCAGAGCC CCTCCCTGCG TGTCCTTCCC CTGGCGGACT GCCTTCTCCC 1861 ACATCGTATC TTTCTAGAAG ATCTCCTACA ATATTCTCAG CTGCCATGGA AAATCGATGT 1921 TCTTCTTTTA TTCTCTCAAG ATTTTCAGGC TGTATATTAA AACTTATATT AAGAACTATG 1981 CTAACCACCT CATCAGGAAC CGTTGTAGGT GGCGTGGGTT TTCTTGGCAA TCGACTCTCA 2041 TGAAAACTAC GAGCTAAATA TTCAATATGT TCCTCTTGAC CAACTTTATT CTGCATTTTT 2101 TTTGAACGAG GTTTAGAGCA AGCTTCAGGA AACTGAGACA GGAATTTTAT TAAAAATTTA 2161 AATTTTGAAG AAAGTTCAGG GTTAATAGCA TCCATTTTTT GCTTTGCAAG TTCCTCAGCA 2221 TTCTTAACAA AAGACGTCTC TTTTGACATG TTTAAAGTTT AAACCTCCTG TGTGAAATTG 2281 TTATCCGCTC ACAATTCCAC ACATTATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 2341 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCAA TTGCTTTCCA 2401 GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG 2461 TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG 2521 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 2581 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 2641 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 2701 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 2761 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 2821 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 2881 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 2941 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 3001 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 3061 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC 3121 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 3181 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 3241 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 3301 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 3361 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 3421 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 3481 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 3541 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 3601 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 3661 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 3721 TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 3781 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 3841 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 3901 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 3961 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 4021 TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 4081 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 4141 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 4201 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 4261 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 4321 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 4381 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT 4441 AACCTATAAA AATAGGCGTA TCACGAGGCC GCCCCTGCAG CCGAATTATA TTATTTTTGC 4501 CAAATAATTT TTAACAAAAG CTCTGAAGTC TTCTTCATTT AAATTCTTAG ATGATACTTC 4561 ATCTGGAAAA TTGTCCCAAT TAGTAGCATC ACGCTGTGAG TAAGTTCTAA ACCATTTTTT 4621 TATTGTTGTA TTATCTCTAA TCTTACTACT CGATGAGTTT TCGGTATTAT CTCTATTTTT 4681 AACTTGGAGC AGGTTCCATT CATTGTTTTT TTCATCATAG TGAATAAAAT CAACTGCTTT 4741 AA //