LOCUS pJET_41_TRE3GS-naobody- 5016 bp ds-DNA circular 19-JUL-2021 DEFINITION . KEYWORDS "Name:pJET_41_TRE3GS-naobody-mCHerry-Rab11b" "Plasmid:pJET_41_TRE3GS-naobody-mCHerry-Rab11b" "Strain/Frozen stock" "Resistance (bacteria)" "Gene or insert" "Owner:Jakub" "Location" "Sub-location" "Location details" "Backbone" "Technical details" "Created by" "3' Cloning site - Enzyme Used" "3' primer" "5' Cloning site - Enzyme Used" "5' primer" FEATURES Location/Qualifiers misc_feature 1492..1517 /label="T7 promoter preceded by 6 arbitrary nucleotides for optimal in vitro transcription" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 1492..1517 /label="F_UNIVERSAL" /note="sequence: AGCTGATAATACGACTCACTATAGGG" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a misc_feature 1518..1818 /label="5HDR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer complement(1795..1818) /label="pJET1.2/blunt 10_mCherry_Rab11B_short REV" /note="sequence: ATCACTGATAGGGAGTAAAGAATTTGTCCTGGCGCTTCCGGCGGGATC" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1819..1823 /label="EcoRI" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer 1819..1842 /label="pJET1.2_11_DOX_NeonG_RAB25_ FWD" /note="sequence: GATCCCGCCGGAAGCGCCAGGACAAATTCTTTACTCCCTATCAGTGAT" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1824..2188 /label="TRE3GS promoter" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(2166..2189) /label="pJET1.2_11_DOX_NeonG_RAB25_ REV" /note="sequence: GCCACTAGTCCCACCCGATCCCATCTTTACGAGGGTAGGAAGTGGTAC" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2189..2189 /label="EcoRI" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature complement(2189..2189) /label="EcoRI" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS 2190..3521 /label="Translation 2190-3521" misc_feature 2193..2205 /label="LinkerS1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer complement(2193..2213) /label="R_uniGSlink_SpeI" /note="sequence: GCCACTAGTCCCACCCGATCC" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 2206..2213 /label="LinkerS1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 2206..2213 /label="ORDER_5HDR" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_recomb 2215..2248 /label="FRT" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 2250..2606 /label="Anti-GFP nanobody_human codon optimized" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 2607..2624 /label="linker XhoI" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 2668..3332 /label="mCherry_optimized" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 3333..3353 /label="linkerS2" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer 3333..3353 /label="F_Seq_uniGSlink_AgeI" /note="sequence: GGAACCGGTGCTGGAAGTGGT" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(3333..3353) /label="R_uniGSlink_AgeI" /note="sequence: ACCACTTCCAGCACCGGTTCC" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a exon 3354..3387 /label="RAB11B-001 Exon 1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 3354..3533 /label="3HDR" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer 3434..3450 /label="R_WPRE" /note="sequence: TGCGGGGAGGCGGCCCAA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(3469..3483) /label="R_Rab11b_short" /note="sequence: TCCGGCCTGGGCCCA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a ORIGIN 1 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 61 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 121 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 181 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 241 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 301 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 361 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 421 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 481 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 541 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 601 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 661 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 721 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 781 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 841 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 901 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 961 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 1021 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA 1081 TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC GCCCCTGCAG CCGAATTATA 1141 TTATTTTTGC CAAATAATTT TTAACAAAAG CTCTGAAGTC TTCTTCATTT AAATTCTTAG 1201 ATGATACTTC ATCTGGAAAA TTGTCCCAAT TAGTAGCATC ACGCTGTGAG TAAGTTCTAA 1261 ACCATTTTTT TATTGTTGTA TTATCTCTAA TCTTACTACT CGATGAGTTT TCGGTATTAT 1321 CTCTATTTTT AACTTGGAGC AGGTTCCATT CATTGTTTTT TTCATCATAG TGAATAAAAT 1381 CAACTGCTTT AACACTTGTG CCTGAACACC ATATCCATCC GGCGTAATAC GACTCACTAT 1441 AGGGAGAGCG GCCGCCAGAT CTTCCGGATG GCTCGAGTTT TTCAGCAAGA TAGCTGATAA 1501 TACGACTCAC TATAGGGCAG TCTTCCGCCC CGCCCTCTTG AGCTCGCCCC TGATAGGCTG 1561 GCGCGTCCGT CACTTCAAAA AGGTCCGCAT TCCTTCCGCC TTTCTCCAGG ACACCGAGGG 1621 CGAGGAGGGT GGTACCAAGC GGCGCCCACC CTCAGAGCAC TACTTCCATC TCTGATTGGC 1681 TTCGCTGGGT GCCCGTCGCT ACTCCACTCG CCGCTGATAG GCCATTCAAC CGCCGCTCTG 1741 CGCGGCGCCG GCTCCGCCCC CGTCGGGTGT TTGTGGTGGG GCTGCGGAGT CGCCGATCCC 1801 GCCGGAAGCG CCAGGACAAA TTCTTTACTC CCTATCAGTG ATAGAGAACG TATGAAGAGT 1861 TTACTCCCTA TCAGTGATAG AGAACGTATG CAGACTTTAC TCCCTATCAG TGATAGAGAA 1921 CGTATAAGGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGACCAGTTT ACTCCCTATC 1981 AGTGATAGAG AACGTATCTA CAGTTTACTC CCTATCAGTG ATAGAGAACG TATATCCAGT 2041 TTACTCCCTA TCAGTGATAG AGAACGTATA AGCTTTGCTT ATGTAAACCA GGGCGCCTAT 2101 AAAAGAGTGC TGATTTTTTG AGTAAACTTC AATTCCACAA CACTTTTGTC TTATACCAAC 2161 TTTCCGTACC ACTTCCTACC CTCGTAAAGA TGGGATCGGG TGGGACTAGT GGCgGAAGTT 2221 CCTATTCtct agaaaGtATA GGAACTTCcA TGGCTCAGGT GCAGCTGGTT GAATCTGGCG 2281 GAGCACTGGT TCAGCCTGGC GGATCTCTGA GACTGTCTTG TGCCGCCTCT GGCTTCCCCG 2341 TGAACCGCTA CAGCATGCGG TGGTATAGAC AGGCCCCTGG CAAAGAACGA GAGTGGGTGG 2401 CCGGAATGTC TAGCGCTGGC GATAGAAGCA GCTACGAGGA CAGCGTGAAG GGCAGATTCA 2461 CCATCAGCAG GGACGACGCC AGAAACACCG TGTACCTGCA GATGAACAGC CTGAAGCCTG 2521 AGGACACCGC CGTGTACTAC TGCAACGTGA ACGTGGGCTT CGAGTACTGG GGCCAGGGAA 2581 CCCAAGTGAC CGTGTCTAGT CCTGACGGAG GCTCGAGCGG AGGCATGGTG TCCAAGGGCG 2641 AAGAGGACAA CATGGCCATC ATCAAAGAAT TCATGCGGTT CAAGGTGCAC ATGGAAGGCA 2701 GCGTGAACGG CCACGAGTTC GAGATTGAAG GCGAAGGCGA GGGCAGACCT TACGAGGGAA 2761 CACAGACCGC CAAGCTGAAA GTGACCAAAG GCGGCCCTCT GCCTTTTGCC TGGGACATTC 2821 TGAGCCCTCA GTTTATGTAC GGCAGCAAGG CCTACGTGAA GCACCCCGCC GATATTCCCG 2881 ACTACCTGAA GCTGAGCTTC CCCGAGGGCT TCAAGTGGGA GAGAGTGATG AACTTCGAGG 2941 ACGGCGGCGT GGTCACCGTG ACTCAAGATA GCTCTCTGCA GGACGGCGAG TTCATCTACA 3001 AAGTGAAGCT GCGGGGCACC AACTTTCCCT CTGATGGCCC CGTGATGCAG AAAAAGACCA 3061 TGGGCTGGGA AGCCAGCAGC GAGAGAATGT ACCCTGAAGA TGGCGCCCTG AAGGGCGAGA 3121 TCAAGCAGCG GCTGAAACTG AAGGATGGCG GCCACTACGA CGCCGAAGTG AAAACCACCT 3181 ACAAGGCCAA GAAACCCGTG CAGCTGCCTG GCGCCTACAA CGTGAACATC AAGCTGGACA 3241 TCACCAGCCA CAACGAGGAC TACACCATCG TGGAACAGTA CGAGAGAGCC GAAGGCAGAC 3301 ACAGCACAGG CGGAATGGAC GAGCTGTACA AGGGAACCGG TGCTGGAAGT GGTACCCGGG 3361 ACGACGAGTA CGACTACCTA TTCAAAGGTG CGGCCGGTGG GGCACAGACG GGCGAAGTCG 3421 TGGCGGCGAG GCGGCGGGCA GACGGGCCAA GGCCGCGCTC CAGGCCGCTG GGCCCAGGCC 3481 GGAGCCCGGG GCTTGGGGCC CGGCGGGTCA GGCAGCCGGG AAGGCCGGAG CCGATCTTTC 3541 TAGAAGATCT CCTACAATAT TCTCAGCTGC CATGGAAAAT CGATGTTCTT CTTTTATTCT 3601 CTCAAGATTT TCAGGCTGTA TATTAAAACT TATATTAAGA ACTATGCTAA CCACCTCATC 3661 AGGAACCGTT GTAGGTGGCG TGGGTTTTCT TGGCAATCGA CTCTCATGAA AACTACGAGC 3721 TAAATATTCA ATATGTTCCT CTTGACCAAC TTTATTCTGC ATTTTTTTTG AACGAGGTTT 3781 AGAGCAAGCT TCAGGAAACT GAGACAGGAA TTTTATTAAA AATTTAAATT TTGAAGAAAG 3841 TTCAGGGTTA ATAGCATCCA TTTTTTGCTT TGCAAGTTCC TCAGCATTCT TAACAAAAGA 3901 CGTCTCTTTT GACATGTTTA AAGTTTAAAC CTCCTGTGTG AAATTGTTAT CCGCTCACAA 3961 TTCCACACAT TATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA 4021 GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCAATTGC TTTCCAGTCG GGAAACCTGT 4081 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 4141 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 4201 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 4261 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 4321 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 4381 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 4441 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 4501 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 4561 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 4621 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 4681 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 4741 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 4801 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 4861 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 4921 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 4981 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGA //