LOCUS pJET1.2/blunt_10_mCherr 4184 bp ds-DNA circular 19-JUL-2021 DEFINITION . KEYWORDS "Name:pJET1.2/blunt 10_mCherry_Rab11B_short" "Plasmid:pJET1.2/blunt 10_mCherry_Rab11B_short" "Strain/Frozen stock" "Resistance (bacteria):Amp" "Gene or insert" "Owner" "Location" "Sub-location" "Location details" "Backbone" "Technical details" "Created by" "3' Cloning site - Enzyme Used" "3' primer" "5' Cloning site - Enzyme Used" "5' primer" FEATURES Location/Qualifiers misc_feature 372..397 /label="T7 promoter preceded by 6 arbitrary nucleotides for optimal in vitro transcription" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 372..701 /label="ORDR 5HDR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 gene 397..1581 /label="RAB11B" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer complement(678..696) /label="98" /note="sequence: tctaGAATTCCTGGCGCTTCCGGCGGG" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer 695..718 /label="97" /note="sequence: tatcGAATTCGACAATGGGATCGGGTGGGACTAG" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a exon 699..1485 /label="Translation 699-1485" misc_feature 702..722 /label="LinkerS1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 702..1451 /label="ORDER_specific" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 723..1430 /label="mCherry_optimized" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 1431..1451 /label="linkerS2" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 exon 1452..1485 /label="RAB11B-001 Exon 1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 1452..1581 /label="ORDER 3HDR" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 ORIGIN 1 GCCCCTGCAG CCGAATTATA TTATTTTTGC CAAATAATTT TTAACAAAAG CTCTGAAGTC 61 TTCTTCATTT AAATTCTTAG ATGATACTTC ATCTGGAAAA TTGTCCCAAT TAGTAGCATC 121 ACGCTGTGAG TAAGTTCTAA ACCATTTTTT TATTGTTGTA TTATCTCTAA TCTTACTACT 181 CGATGAGTTT TCGGTATTAT CTCTATTTTT AACTTGGAGC AGGTTCCATT CATTGTTTTT 241 TTCATCATAG TGAATAAAAT CAACTGCTTT AACACTTGTG CCTGAACACC ATATCCATCC 301 GGCGTAATAC GACTCACTAT AGGGAGAGCG GCCGCCAGAT CTTCCGGATG GCTCGAGTTT 361 TTCAGCAAGA TAGCTGATAA TACGACTCAC TATAGGGCAG TCTTCCGCCC CGCCCTCTTG 421 AGCTCGCCCC TGATAGGCTG GCGCGTCCGT CACTTCAAAA AGGTCCGCAT TCCTTCCGCC 481 TTTCTCCAGG ACACCGAGGG CGAGGAGGGT GGTACCAAGC GGCGCCCACC CTCAGAGCAC 541 TACTTCCATC TCTGATTGGC TTCGCTGGGT GCCCGTCGCT ACTCCACTCG CCGCTGATAG 601 GCCATTCAAC CGCCGCTCTG CGCGGCGCCG GCTCCGCCCC CGTCGGGTGT TTGTGGTGGG 661 GCTGCGGAGT CGCCGATCCC GCCGGAAGCG CCAGGACAAT GGGATCGGGT GGGACTAGTG 721 GCATGGTGTC CAAGGGCGAA GAGGACAACA TGGCCATCAT CAAAGAATTC ATGCGGTTCA 781 AGGTGCACAT GGAAGGCAGC GTGAACGGCC ACGAGTTCGA GATTGAAGGC GAAGGCGAGG 841 GCAGACCTTA CGAGGGAACA CAGACCGCCA AGCTGAAAGT GACCAAAGGC GGCCCTCTGC 901 CTTTTGCCTG GGACATTCTG AGCCCTCAGT TTATGTACGG CAGCAAGGCC TACGTGAAGC 961 ACCCCGCCGA TATTCCCGAC TACCTGAAGC TGAGCTTCCC CGAGGGCTTC AAGTGGGAGA 1021 GAGTGATGAA CTTCGAGGAC GGCGGCGTGG TCACCGTGAC TCAAGATAGC TCTCTGCAGG 1081 ACGGCGAGTT CATCTACAAA GTGAAGCTGC GGGGCACCAA CTTTCCCTCT GATGGCCCCG 1141 TGATGCAGAA AAAGACCATG GGCTGGGAAG CCAGCAGCGA GAGAATGTAC CCTGAAGATG 1201 GCGCCCTGAA GGGCGAGATC AAGCAGCGGC TGAAACTGAA GGATGGCGGC CACTACGACG 1261 CCGAAGTGAA AACCACCTAC AAGGCCAAGA AACCCGTGCA GCTGCCTGGC GCCTACAACG 1321 TGAACATCAA GCTGGACATC ACCAGCCACA ACGAGGACTA CACCATCGTG GAACAGTACG 1381 AGAGAGCCGA AGGCAGACAC AGCACAGGCG GAATGGACGA GCTGTACAAG GGAACCGGTG 1441 CTGGAAGTGG TACCCGGGAC GACGAGTACG ACTACCTATT CAAAGGTGCG GCCGGTGGGG 1501 CACAGACGGG CGAAGTCGTG GCGGCGAGGC GGCGGGCAGA CGGGCCAAGG CCGCGCTCCA 1561 GGCCGCTGGG CCCAGGCCGG AATCTTTCTA GAAGATCTCC TACAATATTC TCAGCTGCCA 1621 TGGAAAATCG ATGTTCTTCT TTTATTCTCT CAAGATTTTC AGGCTGTATA TTAAAACTTA 1681 TATTAAGAAC TATGCTAACC ACCTCATCAG GAACCGTTGT AGGTGGCGTG GGTTTTCTTG 1741 GCAATCGACT CTCATGAAAA CTACGAGCTA AATATTCAAT ATGTTCCTCT TGACCAACTT 1801 TATTCTGCAT TTTTTTTGAA CGAGGTTTAG AGCAAGCTTC AGGAAACTGA GACAGGAATT 1861 TTATTAAAAA TTTAAATTTT GAAGAAAGTT CAGGGTTAAT AGCATCCATT TTTTGCTTTG 1921 CAAGTTCCTC AGCATTCTTA ACAAAAGACG TCTCTTTTGA CATGTTTAAA GTTTAAACCT 1981 CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACATTA TACGAGCCGG AAGCATAAAG 2041 TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG 2101 CCAATTGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC 2161 GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG 2221 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 2281 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC 2341 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG 2401 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC 2461 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC 2521 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT 2581 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC 2641 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA 2701 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA 2761 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA 2821 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA 2881 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG 2941 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 3001 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC 3061 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT 3121 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT 3181 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA 3241 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA 3301 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC 3361 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT 3421 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT 3481 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG 3541 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA 3601 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA 3661 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG 3721 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT 3781 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG 3841 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT 3901 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA 3961 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC 4021 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 4081 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCTA AGAAACCATT 4141 ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA GGCC //