LOCUS pJET_64-SFFV-T2A-BlaR-p 4693 bp ds-DNA circular 19-JUL-2021 DEFINITION . KEYWORDS "Name:pJET_64-SFFV-T2A-BlaR-polyA-Rab11B" "Plasmid:pJET_64-SFFV-T2A-BlaR-polyA-Rab11B" "Strain/Frozen stock" "Resistance (bacteria)" "Gene or insert" "Owner" "Location" "Sub-location" "Location details" "Backbone" "Technical details" "Created by" "3' Cloning site - Enzyme Used" "3' primer" "5' Cloning site - Enzyme Used" "5' primer" FEATURES Location/Qualifiers CDS 15..68 /label="T2A" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd CDS 78..476 /label="BSD" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 CDS 78..476 /label="Translation 78-476" polyA_signal 511..738 /label="bGH-pA" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 749..765 /label="ORDER_specific (Invitrogen optimalizovat!)" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 749..765 /label="linkerS2" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 exon 766..799 /label="RAB11B-001 Exon 1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 766..880 /label="ORDER 3HDR" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 3855..3880 /label="T7 promoter preceded by 6 arbitrary nucleotides for optimal in vitro transcription" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 3855..4184 /label="ORDR 5HDR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 4185..4197 /label="ORDER_specific (Invitrogen optimalizovat!)" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 4185..4197 /label="LinkerS1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer 4202..4230 /label="pCDH SFFV NG2 10 FWD" /note="sequence: AGGACAATGGGATCGGGTGGGACTAGGTAACGCCATTTTGCAAGGCATGGAAAAA" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 promoter 4202..4609 /label="SFFV promoter" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 4668..4682 /label="overlap with BAMH1" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(4674..4693) /label="pCDH SFFV NG2 10 REV" /note="sequence: GACTTCCTCTGCCCTCTCCGCTGCCACTAGTGCTCACCATGGATCCCGGG" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 CDS 4684..4693 /label="mNeonGreen2(1-10)" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 CDS 4684..4693 /label="Translation 4684-4693" ORIGIN 1 CTAGTGGCAG CGGAGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAGAATC 61 CCGGCCCTAG GCTCGAGatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa 121 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag 181 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac 241 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt 301 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac 361 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc 421 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaaTCTA 481 GAGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG 541 TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT 601 CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG 661 GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA CAATAGCAGG CATGCTGGGG 721 ATGCGGTGGG CTCTATGGGT CGACGGAACC GGTGCTGGAA GTGGTACCCG GGACGACGAG 781 TACGACTACC TATTCAAAGG TGCGGCCGGT GGGGCACAGA CGGGCGAAGT CGTGGCGGCG 841 AGGCGGCGGG CAGACGGGCC AAGGCCGCGC TCCAGGCCGC ATCTTTCTAG AAGATCTCCT 901 ACAATATTCT CAGCTGCCAT GGAAAATCGA TGTTCTTCTT TTATTCTCTC AAGATTTTCA 961 GGCTGTATAT TAAAACTTAT ATTAAGAACT ATGCTAACCA CCTCATCAGG AACCGTTGTA 1021 GGTGGCGTGG GTTTTCTTGG CAATCGACTC TCATGAAAAC TACGAGCTAA ATATTCAATA 1081 TGTTCCTCTT GACCAACTTT ATTCTGCATT TTTTTTGAAC GAGGTTTAGA GCAAGCTTCA 1141 GGAAACTGAG ACAGGAATTT TATTAAAAAT TTAAATTTTG AAGAAAGTTC AGGGTTAATA 1201 GCATCCATTT TTTGCTTTGC AAGTTCCTCA GCATTCTTAA CAAAAGACGT CTCTTTTGAC 1261 ATGTTTAAAG TTTAAACCTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACATTAT 1321 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT 1381 AATTGCGTTG CGCTCACTGC CAATTGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA 1441 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC 1501 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC 1561 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 1621 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 1681 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 1741 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 1801 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 1861 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 1921 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 1981 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 2041 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 2101 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 2161 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 2221 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 2281 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 2341 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 2401 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 2461 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 2521 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 2581 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 2641 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 2701 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 2761 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 2821 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 2881 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 2941 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 3001 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 3061 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 3121 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 3181 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 3241 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 3301 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 3361 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 3421 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG 3481 GCCGCCCCTG CAGCCGAATT ATATTATTTT TGCCAAATAA TTTTTAACAA AAGCTCTGAA 3541 GTCTTCTTCA TTTAAATTCT TAGATGATAC TTCATCTGGA AAATTGTCCC AATTAGTAGC 3601 ATCACGCTGT GAGTAAGTTC TAAACCATTT TTTTATTGTT GTATTATCTC TAATCTTACT 3661 ACTCGATGAG TTTTCGGTAT TATCTCTATT TTTAACTTGG AGCAGGTTCC ATTCATTGTT 3721 TTTTTCATCA TAGTGAATAA AATCAACTGC TTTAACACTT GTGCCTGAAC ACCATATCCA 3781 TCCGGCGTAA TACGACTCAC TATAGGGAGA GCGGCCGCCA GATCTTCCGG ATGGCTCGAG 3841 TTTTTCAGCA AGATAGCTGA TAATACGACT CACTATAGGG CAGTCTTCCG CCCCGCCCTC 3901 TTGAGCTCGC CCCTGATAGG CTGGCGCGTC CGTCACTTCA AAAAGGTCCG CATTCCTTCC 3961 GCCTTTCTCC AGGACACCGA GGGCGAGGAG GGTGGTACCA AGCGGCGCCC ACCCTCAGAG 4021 CACTACTTCC ATCTCTGATT GGCTTCGCTG GGTGCCCGTC GCTACTCCAC TCGCCGCTGA 4081 TAGGCCATTC AACCGCCGCT CTGCGCGGCG CCGGCTCCGC CCCCGTCGGG TGTTTGTGGT 4141 GGGGCTGCGG AGTCGCCGAT CCCGCCGGAA GCGCCAGGAC AATGGGATCG GGTGGGACTA 4201 GGTAACGCCA TTTTGCAAGG CATGGAAAAA TACCAAACCA AGAATAGAGA AGTTCAGATC 4261 AAGGGCGGGT ACATGAAAAT AGCTAACGTT GGGCCAAACA GGATATCTGC GGTGAGCAGT 4321 TTCGGCCCCG GCCCGGGGCC AAGAACAGAT GGTCACCGCA GTTTCGGCCC CGGCCCGAGG 4381 CCAAGAACAG ATGGTCCCCA GATATGGCCC AACCCTCAGC AGTTTCTTAA GACCCATCAG 4441 ATGTTTCCAG GCTCCCCCAA GGACCTGAAA TGACCCTGCG CCTTATTTGA ATTAACCAAT 4501 CAGCCTGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTTCCC GAGCTCTATA AAAGAGCTCA 4561 CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ACAGACTGAG TCGCCCGGGG GGGATCTGGA 4621 GCTCTCGAGA ATTCTCACGC GTCTGCAGGA TATCAAGCTT GCGGTACCGC GGGCCCGGGA 4681 TCCATGGTGA GCA //