LOCUS Pjet1.2_14_nan-Cherry-R 4682 bp ds-DNA circular 19-JUL-2021 DEFINITION . KEYWORDS "Name:Pjet1.2 14_nan-Cherry-Rab11B" "Plasmid:Pjet1.2 14_nan-Cherry-Rab11B" "Strain/Frozen stock" "Resistance (bacteria)" "Gene or insert" "Owner" "Location" "Sub-location" "Location details" "Backbone" "Technical details" "Created by" "3' Cloning site - Enzyme Used" "3' primer" "5' Cloning site - Enzyme Used" "5' primer" FEATURES Location/Qualifiers misc_feature 372..397 /label="T7 promoter preceded by 6 arbitrary nucleotides for optimal in vitro transcription" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 372..701 /label="ORDR 5HDR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 372..714 /label="ORDER ALL for 2 independent PCR" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 372..714 /label="11_Rab11B_all_3shorter" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 gene 397..714 /label="RAB11B" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 CDS 699..1953 /label="Translation 699-1953" misc_feature 702..714 /label="ORDER_specific (Invitrogen optimalizovat!)" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 702..714 /label="LinkerS1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 715..722 /label="LinkerS1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 715..722 /label="ORDER_5HDR" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 715..1932 /label="ORDER SPECIFIC" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_recomb 724..757 /label="FRT" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 759..1115 /label="Anti-GFP nanobody_human codon optimized" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1116..1133 /label="linker XhoI" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 1134..1841 /label="mCherry_optimized" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_recomb 1844..1877 /label="FRT" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1879..1926 /label="NeonGreen" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 1929..1932 /label="ORDER_3HDR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 1929..1932 /label="linkerS2" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 1933..1949 /label="ORDER_specific (Invitrogen optimalizovat!)" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 1933..1949 /label="linkerS2" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 gene 1933..2079 /label="RAB11B" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 exon 1950..1983 /label="RAB11B-001 Exon 1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 1950..2079 /label="ORDER 3HDR" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 CDS 1953..2080 /label="Translation 1953-2080" primer 2030..2046 /label="R_WPRE" /note="sequence: TGCGGGGAGGCGGCCCAA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a primer complement(2065..2079) /label="R_Rab11b_short" /note="sequence: TCCGGCCTGGGCCCA" /ApEinfo_revcolor=#f7977a /ApEinfo_fwdcolor=#f7977a ORIGIN 1 GCCCCTGCAG CCGAATTATA TTATTTTTGC CAAATAATTT TTAACAAAAG CTCTGAAGTC 61 TTCTTCATTT AAATTCTTAG ATGATACTTC ATCTGGAAAA TTGTCCCAAT TAGTAGCATC 121 ACGCTGTGAG TAAGTTCTAA ACCATTTTTT TATTGTTGTA TTATCTCTAA TCTTACTACT 181 CGATGAGTTT TCGGTATTAT CTCTATTTTT AACTTGGAGC AGGTTCCATT CATTGTTTTT 241 TTCATCATAG TGAATAAAAT CAACTGCTTT AACACTTGTG CCTGAACACC ATATCCATCC 301 GGCGTAATAC GACTCACTAT AGGGAGAGCG GCCGCCAGAT CTTCCGGATG GCTCGAGTTT 361 TTCAGCAAGA TAGCTGATAA TACGACTCAC TATAGGGCAG TCTTCCGCCC CGCCCTCTTG 421 AGCTCGCCCC TGATAGGCTG GCGCGTCCGT CACTTCAAAA AGGTCCGCAT TCCTTCCGCC 481 TTTCTCCAGG ACACCGAGGG CGAGGAGGGT GGTACCAAGC GGCGCCCACC CTCAGAGCAC 541 TACTTCCATC TCTGATTGGC TTCGCTGGGT GCCCGTCGCT ACTCCACTCG CCGCTGATAG 601 GCCATTCAAC CGCCGCTCTG CGCGGCGCCG GCTCCGCCCC CGTCGGGTGT TTGTGGTGGG 661 GCTGCGGAGT CGCCGATCCC GCCGGAAGCG CCAGGACAAT GGGATCGGGT GGGACTAGTG 721 GCgGAAGTTC CTATTCtcta gaaaGtATAG GAACTTCcAT GGCTCAGGTG CAGCTGGTTG 781 AATCTGGCGG AGCACTGGTT CAGCCTGGCG GATCTCTGAG ACTGTCTTGT GCCGCCTCTG 841 GCTTCCCCGT GAACCGCTAC AGCATGCGGT GGTATAGACA GGCCCCTGGC AAAGAACGAG 901 AGTGGGTGGC CGGAATGTCT AGCGCTGGCG ATAGAAGCAG CTACGAGGAC AGCGTGAAGG 961 GCAGATTCAC CATCAGCAGG GACGACGCCA GAAACACCGT GTACCTGCAG ATGAACAGCC 1021 TGAAGCCTGA GGACACCGCC GTGTACTACT GCAACGTGAA CGTGGGCTTC GAGTACTGGG 1081 GCCAGGGAAC CCAAGTGACC GTGTCTAGTC CTGACGGAGG CTCGAGCGGA GGCATGGTGT 1141 CCAAGGGCGA AGAGGACAAC ATGGCCATCA TCAAAGAATT CATGCGGTTC AAGGTGCACA 1201 TGGAAGGCAG CGTGAACGGC CACGAGTTCG AGATTGAAGG CGAAGGCGAG GGCAGACCTT 1261 ACGAGGGAAC ACAGACCGCC AAGCTGAAAG TGACCAAAGG CGGCCCTCTG CCTTTTGCCT 1321 GGGACATTCT GAGCCCTCAG TTTATGTACG GCAGCAAGGC CTACGTGAAG CACCCCGCCG 1381 ATATTCCCGA CTACCTGAAG CTGAGCTTCC CCGAGGGCTT CAAGTGGGAG AGAGTGATGA 1441 ACTTCGAGGA CGGCGGCGTG GTCACCGTGA CTCAAGATAG CTCTCTGCAG GACGGCGAGT 1501 TCATCTACAA AGTGAAGCTG CGGGGCACCA ACTTTCCCTC TGATGGCCCC GTGATGCAGA 1561 AAAAGACCAT GGGCTGGGAA GCCAGCAGCG AGAGAATGTA CCCTGAAGAT GGCGCCCTGA 1621 AGGGCGAGAT CAAGCAGCGG CTGAAACTGA AGGATGGCGG CCACTACGAC GCCGAAGTGA 1681 AAACCACCTA CAAGGCCAAG AAACCCGTGC AGCTGCCTGG CGCCTACAAC GTGAACATCA 1741 AGCTGGACAT CACCAGCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAGAGAGCCG 1801 AAGGCAGACA CAGCACAGGC GGAATGGACG AGCTGTACAA GccGAAGTTC CTATTCtcta 1861 gaaaGtATAG GAACTTCgAC CGAGCTCAAC TTCAAGGAGT GGCAAAAGGC CTTTACCGAT 1921 ATGATGccGG AACCGGTGCT GGAAGTGGTA CCCGGGACGA CGAGTACGAC TACCTATTCA 1981 AAGGTGCGGC CGGTGGGGCA CAGACGGGCG AAGTCGTGGC GGCGAGGCGG CGGGCAGACG 2041 GGCCAAGGCC GCGCTCCAGG CCGCTGGGCC CAGGCCGGAA TCTTTCTAGA AGATCTCCTA 2101 CAATATTCTC AGCTGCCATG GAAAATCGAT GTTCTTCTTT TATTCTCTCA AGATTTTCAG 2161 GCTGTATATT AAAACTTATA TTAAGAACTA TGCTAACCAC CTCATCAGGA ACCGTTGTAG 2221 GTGGCGTGGG TTTTCTTGGC AATCGACTCT CATGAAAACT ACGAGCTAAA TATTCAATAT 2281 GTTCCTCTTG ACCAACTTTA TTCTGCATTT TTTTTGAACG AGGTTTAGAG CAAGCTTCAG 2341 GAAACTGAGA CAGGAATTTT ATTAAAAATT TAAATTTTGA AGAAAGTTCA GGGTTAATAG 2401 CATCCATTTT TTGCTTTGCA AGTTCCTCAG CATTCTTAAC AAAAGACGTC TCTTTTGACA 2461 TGTTTAAAGT TTAAACCTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACATTATA 2521 CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA 2581 ATTGCGTTGC GCTCACTGCC AATTGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT 2641 TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC 2701 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA 2761 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA 2821 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG 2881 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG 2941 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT 3001 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT 3061 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC 3121 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT 3181 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT 3241 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC 3301 TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA 3361 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT 3421 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT 3481 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 3541 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 3601 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 3661 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 3721 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC 3781 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 3841 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 3901 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 3961 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 4021 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 4081 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 4141 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 4201 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 4261 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 4321 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 4381 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 4441 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 4501 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 4561 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 4621 GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG 4681 CC //