LOCUS pJET27_NG2-minilAA7-Rab 4601 bp ds-DNA circular 19-JUL-2021 DEFINITION . KEYWORDS "Name:pJET27_NG2-minilAA7-Rab11A" "Plasmid:pJET27_NG2-minilAA7-Rab11A" "Strain/Frozen stock" "Resistance (bacteria)" "Gene or insert" "Owner" "Location" "Sub-location" "Location details" "Backbone" "Technical details" "Created by" "3' Cloning site - Enzyme Used" "3' primer" "5' Cloning site - Enzyme Used" "5' primer" FEATURES Location/Qualifiers misc_feature 1..21 /label="linkerS2" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer 1..22 /label="pJET1.2/blunt 07_NG2-Rab11A FWD" /note="sequence: gacccagcagaagacaagctccGGAACCGGTGCTGGAAGTGGTA" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1..336 /label="ORDER_3HDR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 19..24 /label="mut PAM" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 19..55 /label="Rab11exon1" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 3311..3336 /label="T7 promoter preceded by 6 arbitrary nucleotides for optimal in vitro transcription" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 3311..3665 /label="ORDER_5HDR" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 3336..3513 /label="intron" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 CDS join(3642..4601,1..56) /label="Translation 3642-56" misc_feature 3645..3665 /label="LinkerS1" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 3666..4370 /label="mNeonGren_optimized" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer complement(4349..4370) /label="pJET1.2/blunt 07_NG2-Rab11A REV" /note="sequence: cgcctccgccagatccgcctccCTTGTACAGCTCGTCCATGCCC" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer 4371..4392 /label="pSH-EFIRES-P-Seipin-miniIAA7-mScarlet-I FWD" /note="sequence: GGGCATGGACGAGCTGTACAAGggaggcggatctggcggaggcg" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 4398..4601 /label="minilAA7" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer complement(4580..4601) /label="pSH-EFIRES-P-Seipin-miniIAA7-mScarlet-I REV" /note="sequence: TACCACTTCCAGCACCGGTTCCggagcttgtcttctgctgggtc" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 ORIGIN 1 GGAACCGGTG CTGGAAGTGG TACACGCGAC GACGAGTACG ACTACCTCTT TAAAGGTGAG 61 GCCATGGGCT CTCGCACTCT ACACAGTCCT CGTTCGGGGA CCCGGGCCAC TCCCGGTGGA 121 CCCTCGTGCC GGCCACCCCT GCACTGATAT AGGCCTCCCT CAGCCCTTCC TTTTTGTGCG 181 GTTCCGTCTC CTACCCAGCT CAGCCTCTTC TCCCCCGCTC AGACAGGGGT CCCCATCACA 241 TGCCGCTCTC TGAGCGACCT CTCCATAGGC CTTCGCTGGC CTCAGAGCCC CTCCCTGCGT 301 GTCCTTCCCC TGGCGGACTG CCTTCTCCCA CATCGTATCT TTCTAGAAGA TCTCCTACAA 361 TATTCTCAGC TGCCATGGAA AATCGATGTT CTTCTTTTAT TCTCTCAAGA TTTTCAGGCT 421 GTATATTAAA ACTTATATTA AGAACTATGC TAACCACCTC ATCAGGAACC GTTGTAGGTG 481 GCGTGGGTTT TCTTGGCAAT CGACTCTCAT GAAAACTACG AGCTAAATAT TCAATATGTT 541 CCTCTTGACC AACTTTATTC TGCATTTTTT TTGAACGAGG TTTAGAGCAA GCTTCAGGAA 601 ACTGAGACAG GAATTTTATT AAAAATTTAA ATTTTGAAGA AAGTTCAGGG TTAATAGCAT 661 CCATTTTTTG CTTTGCAAGT TCCTCAGCAT TCTTAACAAA AGACGTCTCT TTTGACATGT 721 TTAAAGTTTA AACCTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CATTATACGA 781 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 841 GCGTTGCGCT CACTGCCAAT TGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA 901 TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG 961 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG 1021 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 1081 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 1141 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 1201 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 1261 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 1321 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 1381 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 1441 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 1501 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 1561 ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 1621 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 1681 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 1741 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 1801 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 1861 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA 1921 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG 1981 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA 2041 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT 2101 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 2161 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA 2221 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA 2281 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA 2341 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT 2401 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA 2461 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG 2521 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC 2581 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA 2641 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT 2701 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT 2761 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 2821 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 2881 GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCG 2941 CCCCTGCAGC CGAATTATAT TATTTTTGCC AAATAATTTT TAACAAAAGC TCTGAAGTCT 3001 TCTTCATTTA AATTCTTAGA TGATACTTCA TCTGGAAAAT TGTCCCAATT AGTAGCATCA 3061 CGCTGTGAGT AAGTTCTAAA CCATTTTTTT ATTGTTGTAT TATCTCTAAT CTTACTACTC 3121 GATGAGTTTT CGGTATTATC TCTATTTTTA ACTTGGAGCA GGTTCCATTC ATTGTTTTTT 3181 TCATCATAGT GAATAAAATC AACTGCTTTA ACACTTGTGC CTGAACACCA TATCCATCCG 3241 GCGTAATACG ACTCACTATA GGGAGAGCGG CCGCCAGATC TTCCGGATGG CTCGAGTTTT 3301 TCAGCAAGAT AGCTGATAAT ACGACTCACT ATAGGGACTA TGGTAGCTAG GAGTTCCAGG 3361 ACTCAGTTTC CCCTTTGAGC CTCCTTTAGC GACTAAAGCT TGAAGCCCCA CGCATCTCGA 3421 CTCTCGCGCA CACCGCCCTT GTTGGGCTCA GGGGCGGGGC GCCGCCCCCG GAAGTACTTC 3481 CCCTTAAAGG CTGGGGCCTG CCGGAAATGG CGCAGCGGCA GGGAGGGGCT CTTCACCCAG 3541 TCCGGCAGTT GAAGCTCGGC GCTCGGGTTA CCCCTGCAGC GACGCCCCCT GGTCCCACAG 3601 ATACCACTGC TGCTCCCGCC CTTTCGCTCC TCGGCCGCGC AATGGGATCG GGTGGGACTA 3661 GTGGCGTGTC TAAGGGCGAA GAGGACAATA TGGCCAGCCT GCCTGCCACA CACGAGCTGC 3721 ACATCTTCGG CAGCATCAAC GGCGTGGACT TCGACATGGT CGGACAAGGC ACCGGCAATC 3781 CCAACGACGG CTACGAGGAA CTGAACCTGA AGTCCACCAA GGGCGACCTG CAGTTCAGCC 3841 CCTGGATTCT GGTGCCTCAC ATCGGCTACG GCTTCCACCA GTACCTGCCT TATCCTGACG 3901 GCATGAGCCC TTTCCAGGCC GCCATGGTTG ATGGCTCTGG CTACCAGGTG CACCGGACCA 3961 TGCAGTTTGA AGATGGCGCC AGCCTGACCG TGAACTACCG GTACACATAC GAGGGCAGCC 4021 ACATCAAAGG CGAGGCCCAA GTGAAAGGCA CAGGCTTCCC TGCTGATGGC CCCGTGATGA 4081 CCAATTCTCT GACAGCCGCC GACTGGTGCA GAAGCAAGAA AACATACCCC AACGACAAGA 4141 CCATCATCAG CACCTTCAAG TGGTCCTACA CCACCGGCAA CGGCAAGCGG TACAGAAGCA 4201 CCGCCAGAAC CACCTACACC TTCGCCAAAC CTATGGCCGC CAACTACCTG AAGAACCAGC 4261 CTATGTACGT GTTCCGCAAG ACCGAGCTGA AGCACTCCAA GACAGAACTG AACTTCAAAG 4321 AGTGGCAGAA AGCCTTCACC GACGTGATGG GCATGGACGA GCTGTACAAG ggaggcggat 4381 ctggcggagg cggatccggc ttctctgaga ccgtggacct gatgctgaac ctgcagtcca 4441 ataaggaggg ctctgtggat ctgaagaacg tgagcgccgt gcctaaggag aagaccacac 4501 tgaaggaccc atccaagccc cctgccaagg cacaggtggt gggatggcca cccgtgcgga 4561 actacagaaa gaatatgatg acccagcaga agacaagctc c //