LOCUS pLenti_Lifeact-mTagBFP2 8754 bp ds-DNA circular 25-JAN-2021 DEFINITION . KEYWORDS "Name:pLenti Lifeact-mTagBFP2 PuroR" "Plasmid:pLenti Lifeact-mTagBFP2 PuroR" "Strain/Frozen stock" "Resistance (bacteria)" "Gene or insert" "Owner" "Location" "Sub-location" "Location details" "Backbone" "Technical details" "Created by" "3' Cloning site - Enzyme Used" "3' primer" "5' Cloning site - Enzyme Used" "5' primer" "marker:AmpR, PuroR" FEATURES Location/Qualifiers LTR 1..234 /label="3' LTR (ΔU3)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 polyA_signal 306..427 /label="SV40 poly(A) signal" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac rep_origin 467..602 /label="SV40 ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 promoter complement(623..641) /label="T7 promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind complement(651..667) /label="M13 fwd" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 rep_origin 809..1264 /label="f1 ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 promoter 1290..1394 /label="AmpR promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 1395..1463 /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 CDS 1464..2255 /label="AmpR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 rep_origin 2426..3014 /label="ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 protein_bind 3302..3323 /label="CAP binding site" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b promoter 3338..3343 /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 3344..3361 /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 3362..3368 /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 protein_bind 3376..3392 /label="lac operator" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b primer_bind 3400..3416 /label="M13 rev" /ApEinfo_revcolor=#a020f0 /ApEinfo_fwdcolor=#a020f0 promoter 3437..3455 /label="T3 promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 3483..3709 /label="RSV promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 LTR 3710..3890 /label="5' LTR (truncated)" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 3937..4062 /label="HIV-1 Ψ" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 4555..4788 /label="RRE" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 5284..5400 /label="cPPT/CTS" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 protein_bind 5457..5481 /label="attB1" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b promoter 5597..5800 /label="CMV promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 5877..5886 /label="Kozak sequence" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac CDS 5883..5933 /label="Lifeact" /ApEinfo_revcolor=#993366 /ApEinfo_fwdcolor=#993366 CDS 5883..6665 /label="Translation 5883-6665" misc_feature 5949..5958 /label="Kozak sequence" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 5955..5979 /label="171_F_EcoRI-mTagBFP2" /note="sequence: gataGAATTCAAGatggtgtctaagggcgaagagctga" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc CDS 5955..6665 /label="mTagBFP2" /ApEinfo_revcolor=#3366ff /ApEinfo_fwdcolor=#3366ff CDS 5955..6665 /label="Translation 5955-6665" primer complement(6641..6663) /label="172_R_AgeI-mTagBFP2" /note="sequence: taACCGGTattaagcttgtgccccagtttgcta" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS 6666..6717 /label="Translation 6666-6717" protein_bind 6738..6758 /label="attB5" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b protein_bind complement(6870..6894) /label="attB2" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b misc_feature 6925..7513 /label="WPRE" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS complement(7396..7407) /label="Factor Xa site" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd promoter 7527..8026 /label="PGK promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS 8061..8660 /label="PuroR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 CDS 8061..8660 /label="Translation 8061-8660" ORIGIN 1 tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc 61 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 121 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 181 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 241 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 301 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 361 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 421 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac 481 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 541 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 601 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc 661 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 721 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 781 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg 841 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 901 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 961 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 1021 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 1081 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 1141 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 1201 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca 1261 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 1321 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 1381 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 1441 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 1501 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 1561 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 1621 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 1681 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 1741 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 1801 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 1861 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 1921 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 1981 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 2041 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 2101 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 2161 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 2221 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 2281 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 2341 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 2401 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 2461 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 2521 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 2581 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 2641 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 2701 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 2761 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 2821 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 2881 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 2941 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 3001 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 3061 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 3121 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 3181 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 3241 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 3301 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 3361 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 3421 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt 3481 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca 3541 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga 3601 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt 3661 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg 3721 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 3781 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 3841 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg 3901 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt 3961 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 4021 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 4081 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 4141 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 4201 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 4261 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 4321 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 4381 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga 4441 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 4501 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 4561 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct 4621 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 4681 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 4741 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 4801 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 4861 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa 4921 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 4981 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 5041 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 5101 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 5161 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 5221 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 5281 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 5341 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 5401 atcgtccagt gtggtggaat tgagtattcc agtgtggtgg aattctgcag atatcaacaa 5461 gtttgtacaa aaaagcaggc tggagaccca agcttggtac cgagctcgga tccactagta 5521 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 5581 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 5641 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 5701 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 5761 cggtaggcgt gtacggtggg aggtttatat aagcagagct cgtttagtga accgtcagat 5821 cgcctggaga cgccatccac gctgttttga cctccataga agattctaga gctagcgcca 5881 ccatgggcgt ggccgacttg atcaagaagt tcgagtccat ctccaaggag gagggggatc 5941 caccggtcgc caccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga 6001 agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag 6061 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc 6121 ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc 6181 acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga 6241 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg 6301 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg 6361 tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac cccgctgacg 6421 gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg 6481 caaacgccaa gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg 6541 tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag acctacgtcg 6601 agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc 6661 ttaattaagc ggccgcgact ctagaattct gcagatatcc atcacactgg cggccgctcg 6721 agcatgcatc tagaggacaa ctttgtatac aaaagttggg agacccaagc ttggtaccga 6781 gctcggatcc actagtaacg gccgccagtg tgctggaatt ctgcagatat ccatcacact 6841 ggcggccgct cgagcatgca tctagaggta cccagctttc ttgtacaaag tggttgatat 6901 ccagcacagt ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg 6961 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct 7021 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 7081 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact 7141 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc 7201 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc 7261 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag 7321 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 7381 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg 7441 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg 7501 gccgcctccc cgcctggaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg 7561 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg 7621 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc 7681 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg 7741 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc 7801 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc 7861 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag 7921 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg 7981 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag 8041 cttaccacac tcctgcaggc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg 8101 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc 8161 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc 8221 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg 8281 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc 8341 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg 8401 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc 8461 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg 8521 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc 8581 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga 8641 cccgcaagcc cggtgcctga acgcgtatgc atctcgaggg cccggtacct ttaagaccaa 8701 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggac //