LOCUS pJET_63-SFFV-T2A-BlaR-p 4897 bp ds-DNA circular 01-APR-2022 DEFINITION . KEYWORDS "3' Cloning site - Enzyme Used" "3' primer" "5' Cloning site - Enzyme Used" "5' primer" "Backbone" "Created by" "Gene or insert" "Location" "Location details" "Name:pJET_63-SFFV-T2A-BlaR-polyA-Rab11A" "Owner" "Plasmid:pJET_63-SFFV-T2A-BlaR-polyA-Rab11A_NGS correct" "Resistance (bacteria)" "Strain/Frozen stock" "Sub-location" "Technical details" FEATURES Location/Qualifiers protein_bind 165..186 /label="CAP binding site" promoter 201..231 /label="lac UV5 promoter" misc_feature 666..691 /label="T7 promoter preceded by 6 arbitrary nucleotides for optimal in vitro transcription" promoter 672..690 /label="T7 promoter" misc_feature 691..868 /label="intron" misc_feature 691..996 /label="5HDR" promoter 1017..1424 /label="SFFV promoter" misc_feature 1499..1507 /label="START" exon 1499..1984 /label="Translation 1499-1984" CDS 1523..1576 /label="T2A" CDS 1586..1984 /label="BSD" polyA_signal 2022..2246 /label="bGH poly(A) signal" misc_feature 2271..2307 /label="Rab11exon1" misc_feature 2271..2588 /label="3HDR" promoter complement(2637..2655) /label="T7 promoter" promoter 3047..3151 /label="AmpR promoter" CDS 3152..3220 /label="AmpR" exon 3152..3220 /label="Translation 3152-3220" CDS 3221..4012 /label="AmpR" exon 3221..4012 /label="Translation 3221-4012" rep_origin 4183..4771 /label="ori" ORIGIN 1 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 61 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 121 aggtttcccg actggaaagc aattggcagt gagcgcaacg caattaatgt gagttagctc 181 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggaatt 241 atgagcggat aataatttca cacaggaggt ttaaacttta aacatgtcaa aagagacgtc 301 ttttgttaag aatgctgagg aacttgcaaa gcaaaaaatg gatgctatta accctgaact 361 ttcttcaaaa tttaaatttt taataaaatt cctgtctcag tttcctgaag cttgctctaa 421 acctcgttca aaaaaaatgc agaataaagt tggtcaagag gaacatattg aatatttagc 481 tcgtagtttt catgagagtc gattgccaag aaaacccacg ccacctacaa cggttcctga 541 tgaggtggtt agcatagttc ttaatataag ttttaatata cagcctgaaa atcttgagag 601 aataaaagaa gaacatcgat tttccatggc agctgagaat attgtaggag atcttctaga 661 aagatagctg ataatacgac tcactatagg gactatggta gctaggagtt ccaggactca 721 gtttcccctt tgagcctcct ttagcgacta aagcttgaag ccccacgcat ctcgactctc 781 gcgcacaccg cccttgttgg gctcaggggc ggggcgccgc ccccggaagt acttcccctt 841 aaaggctggg gcctgccgga aatggcgcag cggcagggag gggctcttca cccagtccgg 901 cagttgaagc tcggcgctcg ggttacccct gcagcgacgc cccctggtcc cacagatacc 961 actgctgctc ccgccctttc gctcctcggc cgcgcaATGG GATCGGGTGG GACTAGGTAA 1021 CGCCATTTTG CAAGGCATGG AAAAATACCA AACCAAGAAT AGAGAAGTTC AGATCAAGGG 1081 CGGGTACATG AAAATAGCTA ACGTTGGGCC AAACAGGATA TCTGCGGTGA GCAGTTTCGG 1141 CCCCGGCCCG GGGCCAAGAA CAGATGGTCA CCGCAGTTTC GGCCCCGGCC CGAGGCCAAG 1201 AACAGATGGT CCCCAGATAT GGCCCAACCC TCAGCAGTTT CTTAAGACCC ATCAGATGTT 1261 TCCAGGCTCC CCCAAGGACC TGAAATGACC CTGCGCCTTA TTTGAATTAA CCAATCAGCC 1321 TGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TTCCCGAGCT CTATAAAAGA GCTCACAACC 1381 CCTCACTCGG CGCGCCAGTC CTCCGACAGA CTGAGTCGCC CGGGGGGGAT CTGGAGCTCT 1441 CGAGAATTCT CACGCGTCTG CAGGATATCA AGCTTGCGGT ACCGCGGGCC CGGGATCCAT 1501 GGTGAGCACT AGTGGCAGCG GAGAGGGCAG AGGAAGTCTT CTAACATGCG GTGACGTGGA 1561 GGAGAATCCC GGCCCTAGGC TCGAGatggc caagcctttg tctcaagaag aatccaccct 1621 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc 1681 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac 1741 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa 1801 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg 1861 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga 1921 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg 1981 ctaaTCtaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca 2041 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac 2101 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat 2161 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca 2221 tgctggggat gcggtgggct ctatgggtcg acggaaccgg tgctggaagt ggtacacgcg 2281 acgacgagta cgactacctc tttaaaggtg aggccatggg ctctcgcact ctacacagtc 2341 ctcgttcggg gacccgggcc actcccggtg gaccctcgtg ccggccaccc ctgcactgat 2401 ataggcctcc ctcagccctt cctttttgtg cggttccgtc tcctacccag ctcagcctct 2461 tctcccccgc tcagacaggg gtccccatca catgccgctc tctgagcgac ctctccatag 2521 gccttcgctg gcctcagagc ccctccctgc gtgtccttcc cctggcggac tgccttctcc 2581 cacatcgtat cttgctgaaa aactcgagcc atccggaaga tctggcggcc gctctcccta 2641 tagtgagtcg tattacgccg gatggatatg gtgttcaggc acaagtgtta aagcagttga 2701 ttttattcac tatgatgaaa aaaacaatga atggaacctg ctccaagtta aaaatagaga 2761 taataccgaa aactcatcga gtagtaagat tagagataat acaacaataa aaaaatggtt 2821 tagaacttac tcacagcgtg atgctactaa ttgggacaat tttccagatg aagtatcatc 2881 taagaattta aatgaagaag acttcagagc ttttgttaaa aattatttgg caaaaataat 2941 ataattcggc tgcaggggcg gcctcgtgat acgcctattt ttataggtta atgtcatgat 3001 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 3061 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 3121 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 3181 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 3241 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 3301 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 3361 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 3421 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 3481 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 3541 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 3601 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 3661 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 3721 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 3781 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 3841 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 3901 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 3961 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 4021 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 4081 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 4141 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 4201 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 4261 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 4321 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 4381 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 4441 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 4501 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 4561 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 4621 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 4681 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 4741 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 4801 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 4861 ggataaccgt attaccgcct ttgagtgagc tgatacc //