LOCUS pJET-07-mNeonGreen-R11a 4368 bp ds-DNA circular 01-APR-2022 DEFINITION . KEYWORDS "marker:AmpR" FEATURES Location/Qualifiers protein_bind 810..831 /label="CAP binding site" promoter 846..851 /label="lac UV5 promoter" promoter 846..876 /label="lac UV5 promoter" promoter 852..869 /label="lac UV5 promoter" promoter 870..876 /label="lac UV5 promoter" misc_feature 1311..1336 /label="T7 promoter preceded by 6 arbitrary nucleotides for optimal in vitro transcription" promoter 1317..1335 /label="T7 promoter" misc_feature 1336..1513 /label="intron" misc_feature 1336..1640 /label="5HDR" CDS 1641..2424 /label="Translation 1641-2424" misc_feature 1644..1664 /label="LinkerS1" misc_feature 1665..2369 /label="mNeonGreen" misc_feature 2370..2387 /label="linkerS2" misc_feature 2388..2393 /label="mut PAM" misc_feature 2388..2424 /label="Rab11exon1" misc_feature 2388..2704 /label="3HDR" primer_bind complement(2752..2772) /label="T7" promoter 3163..3267 /label="AmpR promoter" CDS 3268..3336 /label="AmpR" CDS 3337..4128 /label="AmpR" rep_origin join(4299..4368,1..519) /label="ori" ORIGIN 1 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 61 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 121 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 181 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 241 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 301 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 361 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 421 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 481 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 541 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 601 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 661 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg 721 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 781 aaagcaattg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 841 caggctttac actttatgct tccggctcgt ataatgtgtg gaattatgag cggataataa 901 tttcacacag gaggtttaaa ctttaaacat gtcaaaagag acgtcttttg ttaagaatgc 961 tgaggaactt gcaaagcaaa aaatggatgc tattaaccct gaactttctt caaaatttaa 1021 atttttaata aaattcctgt ctcagtttcc tgaagcttgc tctaaacctc gttcaaaaaa 1081 aatgcagaat aaagttggtc aagaggaaca tattgaatat ttagctcgta gttttcatga 1141 gagtcgattg ccaagaaaac ccacgccacc tacaacggtt cctgatgagg tggttagcat 1201 agttcttaat ataagtttta atatacagcc tgaaaatctt gagagaataa aagaagaaca 1261 tcgattttcc atggcagctg agaatattgt aggagatctt ctagaaagat agctgataat 1321 acgactcact atagggacta tggtagctag gagttccagg actcagtttc ccctttgagc 1381 ctcctttagc gactaaagct tgaagcccca cgcatctcga ctctcgcgca caccgccctt 1441 gttgggctca ggggcggggc gccgcccccg gaagtacttc cccttaaagg ctggggcctg 1501 ccggaaatgg cgcagcggca gggaggggct cttcacccag tccggcagtt gaagctcggc 1561 gctcgggtta cccctgcagc gacgccccct ggtcccacag ataccactgc tgctccgccc 1621 tttcgctcct cggccgcgca atgggatcgg gtgggactag tggcgtgtct aagggcgaag 1681 aggacaatat ggccagcctg cctgccacac acgagctgca catcttcggc agcatcaacg 1741 gcgtggactt cgacatggtc ggacaaggca ccggcaatcc caacgacggc tacgaggaac 1801 tgaacctgaa gtccaccaag ggcgacctgc agttcagccc ctggattctg gtgcctcaca 1861 tcggctacgg cttccaccag tacctgcctt atcctgacgg catgagccct ttccaggccg 1921 ccatggttga tggctctggc taccaggtgc accggaccat gcagtttgaa gatggcgcca 1981 gcctgaccgt gaactaccgg tacacatacg agggcagcca catcaaaggc gaggcccaag 2041 tgaaaggcac aggcttccct gctgatggcc ccgtgatgac caattctctg acagccgccg 2101 actggtgcag aagcaagaaa acatacccca acgacaagac catcatcagc accttcaagt 2161 ggtcctacac caccggcaac ggcaagcggt acagaagcac cgccagaacc acctacacct 2221 tcgccaaacc tatggccgcc aactacctga agaaccagcc tatgtacgtg ttccgcaaga 2281 ccgagctgaa gcactccaag acagaactga acttcaaaga gtggcagaaa gccttcaccg 2341 acgtgatggg catggacgag ctgtacaagg gaaccggtgc tggaagtggt acacgcgacg 2401 acgagtacga ctacctcttt aaaggtgagg ccatgggctc tcgcactcta cacagtcctc 2461 gttcggggac ccgggccact cccggtggac cctcgtgccg gccacccctg cactgatata 2521 ggcctccctc agcccttcct ttttgtgcgg ttccgtctcc tacccagctc agcctcttct 2581 cccccgctca gacaggggtc cccatcacat gccgctctct gagcgacctc tccataggcc 2641 ttcgctggcc tcagagcccc tccctgcgtg tccttcccct ggcggactgc cttctcccac 2701 atcgatcttg ctgaaaaact cgagccatcc ggaagatctg gcggccgctc tccctatagt 2761 gagtcgtatt acgccggatg gatatggtgt tcaggcacaa gtgttaaagc agttgatttt 2821 attcactatg atgaaaaaaa caatgaatgg aacctgctcc aagttaaaaa tagagataat 2881 accgaaaact catcgagtag taagattaga gataatacaa caataaaaaa atggtttaga 2941 acttactcac agcgtgatgc tactaattgg gacaattttc cagatgaagt atcatctaag 3001 aatttaaatg aagaagactt cagagctttt gttaaaaatt atttggcaaa aataatataa 3061 ttcggctgca ggggcggcct cgtgatacgc ctatttttat aggttaatgt catgataata 3121 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt 3181 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg 3241 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt 3301 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta 3361 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc 3421 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa 3481 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc 3541 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt 3601 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact 3661 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac 3721 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata 3781 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta 3841 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg 3901 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat 3961 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt 4021 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga 4081 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa 4141 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag 4201 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac 4261 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 4321 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtt //