LOCUS pJET-22-DExCon-mNeonGre 4740 bp ds-DNA circular 01-APR-2022 DEFINITION . KEYWORDS "marker:AmpR" FEATURES Location/Qualifiers promoter 1..31 /label="lac UV5 promoter" promoter 472..490 /label="T7 promoter" misc_feature 491..668 /label="intron" misc_feature 491..795 /label="5HDR" misc_feature 802..1166 /label="TRE3GS promoter" protein_bind 807..825 /label="tet operator" protein_bind 843..861 /label="tet operator" protein_bind 879..897 /label="tet operator" protein_bind 915..933 /label="tet operator" protein_bind 951..969 /label="tet operator" protein_bind 987..1005 /label="tet operator" protein_bind 1023..1041 /label="tet operator" CDS 1168..1951 /label="Translation 1168-1951" misc_feature 1171..1191 /label="LinkerS1" misc_feature 1192..1896 /label="mNeonGreen" misc_feature 1897..1914 /label="linkerS2" misc_feature 1915..1920 /label="mut PAM" misc_feature 1915..1951 /label="Rab11exon1" misc_feature 1915..2231 /label="3HDR" primer_bind complement(2279..2299) /label="T7" promoter 2690..2794 /label="AmpR promoter" CDS 2795..2863 /label="AmpR" CDS 2864..3655 /label="AmpR" rep_origin 3826..4414 /label="ori" protein_bind 4705..4726 /label="CAP binding site" ORIGIN 1 tttacacttt atgcttccgg ctcgtataat gtgtggaatt atgagcggat aataatttca 61 cacaggaggt ttaaacttta aacatgtcaa aagagacgtc ttttgttaag aatgctgagg 121 aacttgcaaa gcaaaaaatg gatgctatta accctgaact ttcttcaaaa tttaaatttt 181 taataaaatt cctgtctcag tttcctgaag cttgctctaa acctcgttca aaaaaaatgc 241 agaataaagt tggtcaagag gaacatattg aatatttagc tcgtagtttt catgagagtc 301 gattgccaag aaaacccacg ccacctacaa cggttcctga tgaggtggtt agcatagttc 361 ttaatataag ttttaatata cagcctgaaa atcttgagag aataaaagaa gaacatcgat 421 tttccatggc agctgagaat attgtaggag atcttctaga aagatagctg ataatacgac 481 tcactatagg gactatggta gctaggagtt ccaggactca gtttcccctt tgagcctcct 541 ttagcgacta aagcttgaag ccccacgcat ctcgactctc gcgcacaccg cccttgttgg 601 gctcaggggc ggggcgccgc ccccggaagt acttcccctt aaaggctggg gcctgccgga 661 aatggcgcag cggcagggag gggctcttca cccagtccgg cagttgaagc tcggcgctcg 721 ggttacccct gcagcgacgc cccctggtcc cacagatacc actgctgctc cgccctttcg 781 ctcctcggcc gcgcagaatt ctttactccc tatcagtgat agagaacgta tgaagagttt 841 actccctatc agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg 901 tataaggagt ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag 961 tgatagagaa cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt 1021 actccctatc agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa 1081 aagagtgctg attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt 1141 tccgtaccac ttcctaccct cgtaaagatg ggatcgggtg ggactagtgg cgtgtctaag 1201 ggcgaagagg acaatatggc cagcctgcct gccacacacg agctgcacat cttcggcagc 1261 atcaacggcg tggacttcga catggtcgga caaggcaccg gcaatcccaa cgacggctac 1321 gaggaactga acctgaagtc caccaagggc gacctgcagt tcagcccctg gattctggtg 1381 cctcacatcg gctacggctt ccaccagtac ctgccttatc ctgacggcat gagccctttc 1441 caggccgcca tggttgatgg ctctggctac caggtgcacc ggaccatgca gtttgaagat 1501 ggcgccagcc tgaccgtgaa ctaccggtac acatacgagg gcagccacat caaaggcgag 1561 gcccaagtga aaggcacagg cttccctgct gatggccccg tgatgaccaa ttctctgaca 1621 gccgccgact ggtgcagaag caagaaaaca taccccaacg acaagaccat catcagcacc 1681 ttcaagtggt cctacaccac cggcaacggc aagcggtaca gaagcaccgc cagaaccacc 1741 tacaccttcg ccaaacctat ggccgccaac tacctgaaga accagcctat gtacgtgttc 1801 cgcaagaccg agctgaagca ctccaagaca gaactgaact tcaaagagtg gcagaaagcc 1861 ttcaccgacg tgatgggcat ggacgagctg tacaagggaa ccggtgctgg aagtggtaca 1921 cgcgacgacg agtacgacta cctctttaaa ggtgaggcca tgggctctcg cactctacac 1981 agtcctcgtt cggggacccg ggccactccc ggtggaccct cgtgccggcc acccctgcac 2041 tgatataggc ctccctcagc ccttcctttt tgtgcggttc cgtctcctac ccagctcagc 2101 ctcttctccc ccgctcagac aggggtcccc atcacatgcc gctctctgag cgacctctcc 2161 ataggccttc gctggcctca gagcccctcc ctgcgtgtcc ttcccctggc ggactgcctt 2221 ctcccacatc gatcttgctg aaaaactcga gccatccgga agatctggcg gccgctctcc 2281 ctatagtgag tcgtattacg ccggatggat atggtgttca ggcacaagtg ttaaagcagt 2341 tgattttatt cactatgatg aaaaaaacaa tgaatggaac ctgctccaag ttaaaaatag 2401 agataatacc gaaaactcat cgagtagtaa gattagagat aatacaacaa taaaaaaatg 2461 gtttagaact tactcacagc gtgatgctac taattgggac aattttccag atgaagtatc 2521 atctaagaat ttaaatgaag aagacttcag agcttttgtt aaaaattatt tggcaaaaat 2581 aatataattc ggctgcaggg gcggcctcgt gatacgccta tttttatagg ttaatgtcat 2641 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 2701 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 2761 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 2821 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 2881 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 2941 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 3001 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact 3061 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 3121 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 3181 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 3241 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 3301 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 3361 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 3421 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 3481 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 3541 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 3601 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 3661 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 3721 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 3781 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 3841 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 3901 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 3961 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 4021 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 4081 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 4141 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 4201 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 4261 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 4321 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 4381 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 4441 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 4501 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 4561 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct 4621 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 4681 aattggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc //